Journal: ACS Omega

Article Title: Zinc Oleate Nanorod-Induced Vertical Alignment of Nematic Liquid Crystal

doi: 10.1021/acsomega.2c05196

Figure Lengend Snippet: (left panel) FCM images and (right panel) analysis of FCM images of the (a) sample cell prepared by ZOR-coated ITO substrate and then filled with 8CB and (b) 0.05, (c) 0.3, (d) 2 wt % ZOR nanocomposites of 8CB ( T = 35 °C) in planar cells. (e) FCM image of pure 8CB showing no fluorescence.

Article Snippet: As added NPs do fluoresce, fluorescence confocal microscopy (FCM) (ZEISS LSM880) with laser of wavelength λ = 370 nm is performed to investigate the bulk and interfacial properties of ZOR in the host LC.

Techniques: Fluorescence

Journal: JCI Insight

Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

doi: 10.1172/jci.insight.86492

Figure Lengend Snippet: (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

Techniques: Fluorescence, Microscopy

Journal: JCI Insight

Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

doi: 10.1172/jci.insight.86492

Figure Lengend Snippet: (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

Techniques: Control, Derivative Assay, Fluorescence, Confocal Microscopy, Cell Counting